Eli K. Moore
I am interested in the interaction between microbial and algal communities with the environment and their impact on biogeochemical cycling. Climate sensitive regions and microbial taxa with unique metabolic capabilities are particularly exciting because they can exhibit signs of global change and adaptation. Form and function are intrinsically linked in the world of biochemistry. Uncovering the chemical structure of microbial cellular machinery and its transformation in the environment is crucial in understanding biological function, diagenetic processes, biogeochemical cycling, and climate impacts. I enjoy using high powered analytical techniques such as mass spectrometry, proteomics, and NMR to investigate the biogeochemistry of ecosystems and microbial communities. These techniques can be used to identify complex organic molecules, such as membrane lipids or proteins, in environmental samples in order to understand how microbial communities are adapted to their unique niche or reconstruct past communities from preserved material.
During my PhD work at the University of Maryland I studied protein cycling in the Bering Sea, and the longevity of algal proteins in the water column and sediments. In my postdoc at the Royal Netherlands Institute for sea research my colleagues and I discovered new membrane lipid structures and tracked their distribution in the environment. Now at Rutgers I am exploring protein structure evolution and how it has been influenced by the geosphere in deep time.
Moore EK, Hopmans EC, Rijpstra WIC, Villanueva L, Schoutsen F, Ayden R, Stams F, Sinninghe Damsté JS. 2016. Identification of amino acid containing intact polar membrane lipids through novel fragmentation pathways using liquid chromatography-high resolution mass spectrometry. Rapid Communications in Mass spectrometry, 30, 739-750.
Moore EK, Villanueva L, Hopmans EC, Mets A, Rijpstra WIC, Dedysh SN, Sinninghe Damsté JS. 2015. Abundant Trimethylornithine Lipids and Specific Gene Sequences are Indicative of Planctomycete Importance at the Oxic/Anoxic Interface in Sphagnum-Dominated Northern Wetlands. Applied and Environmental Microbiology, 81, 6333-6344.
Moore EK, Hopmans EC, Rijpstra WIC, Villanueva L, Wienk H, Schoutsen F, Ayden R, Stams F, Sinninghe Damsté JS. 2015. Lysine and novel hydroxylysine lipids in soil bacteria: amino acid membrane lipid response to temperature and pH in Pseudopedobacter saltans. Frontiers in Microbiology, 6, 637.
Moore EK, Harvey HR, Faux JF, Goodlett DR, Nunn BL. 2014. Electrophoreticextraction and proteomic characterization of proteins buried in marine sediments. Chromatography, 1, 176-193. *Invited author to Bioseparations theme issue
Moore EK, Harvey HR, Faux JF, Goodlett DR, Nunn BL. 2014. Protein recycling in Bering Sea algal incubations. Marine Ecology Progress Series, 515, 45-59.
Moore EK, Hopmans EC, Rijpstra WIC,Villanueva L, Dedysh SN, Kulichevskaya IS, Wienk H, Schoutsen F, Sinninghe Damsté JS. 2013. Novel mono-, di-, and trimethylornithine membrane lipids in northern wetland planctomycetes. Applied and Environmental Microbiology, 79, 6874-6884.
Moore EK, Nunn BL, Goodlett DR, Harvey HR. 2012. Identifying and tracking proteins through the marine water column: Insights into the inputs and preservation mechanisms of protein in sediments. Geochimica et Cosmochimica Acta, 83, 324-359.
Moore EK, Nunn BL, Faux JF, Goodlett DR, Harvey HR. 2012. Evaluation of electrophoretic protein extraction and database-driven protein identification from marine sediments. Limnology and Oceanography: Methods, 10, 353-366.