rulogo2.gif (3256 bytes)
Lynx users, please scroll past our index to get to major
content.

Institute of Marine and Coastal Science building (35201 bytes)
Institute of 
Marine & Coastal Sciences

Program Overview:
bullet.gif (825 bytes)Introduction

Internal Methods
and Procedures:
bullet.gif (825 bytes)Methods

Research Group:
bullet.gif (825 bytes)Group Faculty/Staff Directory

Publications:
bullet.gif (825 bytes)Publicized Papers, Manuscripts, Articles, etc. (also ones submitted or in press)

Related Sites:
bullet.gif (825 bytes)Ocean Primary Productivity Study, IMCS, Rutgers Univ.
bullet.gif (825 bytes)Coastal Ocean Observation Laboratory, IMCS, Rutgers Univ.
bullet.gif (825 bytes)The Dismukes Group, Princeton Univ.
bullet.gif (825 bytes)U.S. Joint Global Ocean Flux Study (JGOFS)
bullet.gif (825 bytes)SeaWiFS Project
bullet.gif (825 bytes)MODIS

Funded Projects:
bullet.gif (825 bytes)LIFT/LIDAR - NASA
bullet.gif (825 bytes)Biocomplexity (EREUPT) - NSF
bullet.gif (825 bytes)Cell Death - NIH
bullet.gif (825 bytes)GFP Project - ONR
bullet.gif (825 bytes)Ocean Productivity - NASA
bullet.gif (825 bytes)Iron Limitation - NSF
bullet.gif (825 bytes)IR-FRR - NSF
bullet.gif (825 bytes)Inorganic Carbon Cycling - US/Israel BSF
bullet.gif (825 bytes)Light Intensity - DOE
bullet.gif (825 bytes)Carbon Sequestration - LBNL/DOE
bullet.gif (825 bytes)Benthic Targets (CoBOP) - ONR
bullet.gif (825 bytes)SoFeX - NSF
bullet.gif (825 bytes)Key Phytoplankton Groups (SMP) - NASA
bullet.gif (825 bytes)Photoacclimation (US/Czech Republic) - NSF

Jump to:
bullet.gif (825 bytes) Institute Information
bullet.gif (825 bytes) Upcoming Seminars
bullet.gif (825 bytes) Graduate Program in Oceanography

Special Programs of IMCS:
bullet.gif (825 bytes)Project Tomorrow -K-12 Educational Programs
bullet.gif (825 bytes)
National Undersea Research Program
bullet.gif (825 bytes)New Jersey's Shoreline Future - NJ Shore Protection Master Plan

IMCS Research Facilities:
bullet.gif (825 bytes)Rutgers Marine Field Station in Tuckerton
bullet.gif (825 bytes)
Flume and Seawater Facility
bullet.gif (825 bytes)Haskin Shellfish Research Lab
bullet.gif (825 bytes)
Pinelands Research Station

Find:
People
Places
Events

Search:
Rutgers INFOdex
Rutgers Web sites
The Web

Contact e-mail
romana@imcs.rutgers.edu

Last updated: 07-12-01

The Environmental
Biophysics & Molecular
Ecology Program

   
Funded Current Projects

Back

SERDP Conservation SI-1334

 Analysis of Biophysical, Optical and Genetic Diversity of DoD Coral Reef Communities using Advanced Fluorescence and Molecular Biology Techniques

Sponsored by: Strategic Environmental Research and Development Program (SERDP)
Principal Investigators: Maxim Gorbunov and Paul Falkowski

Background:

 The U.S. Department of Defense (DoD) maintains numerous facilities in tropical and subtropical environments that are adjacent to coral reefs.  Coral reefs are specifically susceptible to anthropogenic insult and rapidly degrade worldwide.  The development of advanced technologies for environmental monitoring of benthic communities under DoD jurisdiction requires an understanding of how different environmental factors affect the key elements of the ecosystems and the selection of specific monitoring protocols that are most appropriate for the identification and quantification of particular stresses.  Documenting the environmental state of reef communities is critical to developing remediation strategies that can both reduce anthropogenic impact and distinguish between natural stress and anthropogenic factors potentially related to military activity.  The quantitative assessment of the impact of stresses requires the accurate knowledge of baseline biophysical, optical, and genetic parameters for “healthy” coral reef communities.  Due to natural variability within populations and diversity amongst species, these parameters vary both within and between species. 

 The Objectives of this SERDP project are  

·        to develop advanced techniques for rapid and non-destructive assessment of the viability and health of coral reef communities with the capabilities of identification and quantification of natural and anthropogenic stresses; 

·        to develop prototypes of Fluorescence Induction and Relaxation (FIRe) Fluorosensors for permanent underwater monitoring stations and Remote Operated Vehicles or Diver Propulsion Vehicles; 

·        to collect an extensive library of baseline data on physiological, biophysical, bio-optical and genetic diversity of coral reef communities near DoD installations. 

 Summary:

 Rapid and non-destructive assessment of the health and viability of benthic photosynthetic organisms is based on the use of variable fluorescence technique.  This technique relies on the relationship between the efficiency of photosynthetic processes and chlorophyll fluorescence and derives a comprehensive suite of fluorescent and photosynthetic parameters of the target (Kolber et al. 1998; Gorbunov and Falkowski 2005).  In laboratory and field experiments, we study the impact of common natural stresses (elevated temperature, excess irradiance, and nutrient load), and selected anthropogenic stresses (like toxic pollutants) on the physiological status of coral.  Our results provide scientific background for the development of advanced protocols for non-destructive assessment of the health of coral reef ecosystems.  The molecular biology and genetic part of the project is focused on identification, as well as spectroscopic and genetic characterization of color proteins from corals.  This information will provide insight into the remarkable diversity of the color palette of reef corals, which is an important indicator of the health of coral reef ecosystems. The instrument development includes design and construction of advanced FIRe systems for permanent monitoring stations and autonomous vehicles.

 
Non-destructive assessment of the health of coral in situ by using an underwater fluorometer
 


Bench-top versions of the Fluorescence Induction and Relaxation (FIRe) System. (Left) – Rutgers FIRe System prototype developed in 2003-2004.  (Right) - commercial FIRe Fluorometer System manufactured by Satlantic Inc.
( see
www.satlantic.com/fire  )

  

 Coral cultivation facilities in Coral Laboratory at the Institute of Marine and Coastal Sciences at Rutgers

 References:  

·        Tchernov D, Gorbunov MY, de Vargas C, Yadav SN, Milligan AJ, Haggblom M, Falkowski PG. (2004) Membrane lipids of symbiotic algae are diagnostic of sensitivity to thermal bleaching in corals. – Proc. Nat. Acad. Sci., U.S.A., 101 (37): 13531-13535.

·        Gorbunov MY, and Falkowski PG. (2005) Fluorescence Induction and Relaxation (FIRe) Technique and Instrumentation for Monitoring Photosynthetic Processes and Primary Production in Aquatic Ecosystems. In: “Photosynthesis: Fundamental Aspects to Global Perspectives” - Proc. 13th International Congress of Photosynthesis, Montreal, Aug.29 – Sept. 3, 2004. (Eds: A. van der Est and D. Bruce), Allen Press, V.2, pp. 1029-1031.


Contact Information:

Dr Maxim Gorbunov
Institute of Marine and Coastal Sciences
Rutgers, the State University of New Jersey
71 Dudley Road, New Brunswick, NJ 08901
Phone: (732) 932 6555, x. 266
Fax: (732) 932 4083
E-mail:  gorbunov@marine.rutgers.edu  

 

[Blueline]

BACK HOME

Environmental Biophysics and Molecular Ecology Program
Institute of Marine and Coastal Sciences
Rutgers, The State University of New Jersey
71 Dudley Road
New Brunswick, NJ 08901
Phone: (732) 932-6555
Fax: (732) 932-4083

© 2006 Rutgers, The State University of New Jersey.
All rights reserved.